CASE REPORT Ongoing twin pregnancy after transfer of vitrified oocyte injected with sperm recovered from cryopreserved testicular tissue

Cryopreservation of human gametes and embryos has become an essential part of assisted reproduction. It is now possible to cryopreserve gametes and embryos at their different stages of development. It allows patients undergoing chemotherapy or radiotherapy to preserve their fertility, and helps to attain all benefits from the costly ovarian superovulation therapies prior to assisted reproductive techniques (ART). Owing to the extremely high survival and pregnancy rates, vitrification is now being widely used in ART laboratories in addition to its low cost and simplicity as an optimal cryopreservation procedure for human oocytes and embryos. Here, we would like to report an ongoing twin pregnancy after Vitrification/warming of oocytes which were injected by ICSI with patient’s husband spermatozoa retrieved from cryopreserved testicular tissue and transfer of day three cleavage stage embryos was.